Rapid species determination of Nocardia keratitis using pyrosequencing technology.

Abstract

To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species. Interventional case report. The medical records of a patient presenting with an infectious keratitis were reviewed. A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring. Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.