Mining a yeast library for brain endothelial cell-binding antibodies.
basic_science · Level V
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- Record sourced from PubMed, PMID 17206151.
- Also identified by PMC identifier 2637222.
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Abstract
We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (K(d) = 82 +/- 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, we used a new yeast display immunoprecipitation procedure for initial characterization of the cognate antigens.
Medical subject headings
- Brain
- Endothelial Cells
- Immunoglobulin Fragments
- Immunoglobulin Variable Region
- Peptide Library
- Saccharomyces cerevisiae