High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 17225864.
- Also identified by PMC identifier PMC117205.
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Abstract
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.