The effects of benzodiazepines on orexinergic systems in rat cerebrocortical slices.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 17242090.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
As orexinergic (OXergic) neurons have been reported to mediate emotional changes, benzodiazepines might interact with OXergic neurons. We examined the interactions between OXergic neurons and benzodiazepine receptors in orexin-A (100 nM) and K+ (25 mM)-evoked norepinephrine release from rat cerebrocortical slices. Midazolam, diazepam, and flunitrazepam concentration-dependently inhibited both OX-A- and K+-evoked norepinephrine release. The IC50 of midazolam for orexin-A-evoked release (0.87 microM, P < 0.01), which was insensitive to flumazenil, was significantly lower than that of diazepam and flunitrazepam (around 60 microM), whereas the IC50s for K+-evoked release were not different among the benzodiazepines. There may be no interaction between OXergic neurons and central benzodiazepine receptors.
Medical subject headings
- Benzodiazepines
- Cerebral Cortex
- Intracellular Signaling Peptides and Proteins
- Neurons
- Neuropeptides