Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
basic_science · Level V
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Abstract
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Medical subject headings
- Biosensing Techniques
- Brain
- Calcium
- Calcium Signaling
- Neurons
- Troponin C