Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting.
basic_science · Level V
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Abstract
New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.
Medical subject headings
- Cell Physiological Phenomena
- Electrophoresis, Gel, Two-Dimensional
- Immunoassay
- Microscopy, Fluorescence
- Protein Interaction Mapping
- Quantum Dots