High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy.
basic_science · Level V
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Abstract
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy, Fluorescence