Reporter proteins for in vivo fluorescence without oxygen.
basic_science · Level V
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- Record sourced from PubMed, PMID 17351616.
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Abstract
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.
Medical subject headings
- Genes, Reporter
- Luminescent Proteins
- Oxygen