Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells.
basic_science · Level V
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Abstract
A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral zeta potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.
Medical subject headings
- DNA
- Drug Delivery Systems
- Endothelial Cells
- Gene Transfer Techniques
- Nanoparticles
- Peptides
- Transfection