Subunit counting in membrane-bound proteins.
basic_science · Level V
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- Record sourced from PubMed, PMID 17369835.
- Also identified by PMC identifier 2744285.
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Abstract
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.
Medical subject headings
- Membrane Proteins
- Protein Subunits
- Recombinant Fusion Proteins