Multiplexed protein detection by proximity ligation for cancer biomarker validation.
other · Level V
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Abstract
We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-microl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.
Medical subject headings
- Antibodies
- Biomarkers, Tumor
- Neoplasm Proteins
- Neoplasms
- Reverse Transcriptase Polymerase Chain Reaction