Non-fouling microfluidic chip produced by radio frequency tetraglyme plasma deposition.

Salim, Malinda; Mishra, Gautam; Fowler, Gregory J S; O'sullivan, Brian; Wright, Phillip C; McArthur, Sally L · Lab Chip · 2007

basic_science · Level V

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Abstract

This Technical Note presents the direct surface modification of a glass/PTFE hybrid microfluidic chip, via radio frequency glow discharge plasma polymerisation of tetraethlylene glycol dimethylether (tetraglyme), to produce hydrophilic, non-fouling, PEO-like surfaces. We use several techniques including X-ray photoelectron spectroscopy (XPS), direct enzyme-linked immunosorbent assays (ELISA) and immunofluorescent imaging to investigate the channel coatings. Our results indicate the successful deposition of a PEO-like coating onto microchannel surfaces that has both solution and shelf stability (>3 months) and is capable of preventing fibrinogen adsorption to the microchannel surfaces.

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