Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10.

Sung, P; Prakash, L; Prakash, S · Nature · 1992

basic_science · Level V

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Abstract

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA, and it functions in mitotic recombination. RAD10 has homology to the human excision repair gene ERCC-1. Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD10 promotes the renaturation of complementary DNA strands.

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