A model system for analyzing somatic mutations in Drosophila melanogaster.

Garcia, Ana Maria; Derventzi, Anastasia; Busuttil, Rita; Calder, R Brent; Perez, Ernesto; Chadwell, Linda; Dollé, Martijn E T; Lundell, Martha et al. · Nat Methods · 2007

basic_science · Level V

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Abstract

Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.

Medical subject headings