A yeast two-hybrid smart-pool-array system for protein-interaction mapping.
basic_science · Level V
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- Record sourced from PubMed, PMID 17450148.
- Also identified by PMC identifier 2771206.
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Abstract
We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.
Medical subject headings
- Protein Interaction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Two-Hybrid System Techniques