Liver and lung late alterations following hepatic reperfusion associated to ischemic preconditioning or N-acetylcysteine.

Abstract

This study aimed the effect of n-acetylcysteine or ischemic preconditioning in hepatic and pulmonary damage after liver ischemia-reperfusion injury. Twenty-four male Wistar-EPM rats were assigned into four groups: (IR) Hepatic ischemia-reperfusion; (IPC) IPC achieved before hepatic ischemia; (NAC) Animals received NAC pretreatment; and Sham operated group. After 24 h of hepatic reperfusion, blood, liver, and pulmonary samples were evaluated. Nonparametric tests were used (P <or= 0.05). Aspartate aminotransferase levels were similar among experimental groups. Lower alanine aminotrasnferase levels were observed in sham group (P = 0.04). IPC and NAC groups prevented from necrosis (P = 0.027), apoptosis (P = 0.003), and microvesicular steatosis (P = 0.0007), but not from neutrophil infiltration in liver tissue. IPC and NAC treatment reduced alveolar septal edema (P = 0.014), but did not prevent from neutrophil infiltration or vascular congestion. In conclusion, IPC and NAC attenuated hepatic and pulmonary damage after hepatic ischemia-reperfusion injury.