SP1 as a novel scaffold building block for self-assembly nanofabrication of submicron enzymatic structures.
basic_science · Level V
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Abstract
In this study, SP1, a ring-shaped highly stable homododecamer protein complex was utilized for the self-assembly of multiple domains in a predefined manner. Glucose oxidase (GOx) was fused in-frame to SP1 and expressed in Escherichia coli. Complexes where GOx encircled SP1 dodecamer were observed, and moreover, the enzymatic monomers self-assembled into active multienzyme nanotube particles containing hundreds of GOx molecules per tube. This work demonstrates the value of SP1 as a self-assembly scaffold.
Medical subject headings
- Drosophila Proteins
- Glucose Oxidase
- Nanostructures
- Nanotechnology
- Sp1 Transcription Factor