Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.
basic_science · Level V
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Abstract
We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.
Medical subject headings
- Image Processing, Computer-Assisted
- Microscopy, Ultraviolet
- Nucleic Acids
- Proteins