LambdaN-GFP: an RNA reporter system for live-cell imaging.
basic_science · Level V
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- Record sourced from PubMed, PMID 17603490.
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Abstract
We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.
Medical subject headings
- Genes, Reporter
- Green Fluorescent Proteins
- RNA