Optical detection of ion-channel-induced proton transport in supported phospholipid bilayers.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 17629349.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The integration of ion-channel transport functions with responses derived from nanostructured and nanoporous silica mesophase materials is demonstrated. Patterned thin-film mesophases consisting of alternating hydrophilic nanoporous regions and hydrophobic nanostructured regions allow for spatially localized proton transport via selective dimerization of gramicidin in lipid bilayers formed on the hydrophilic regions. The adjoining hydrophobic mesostructure doped with a pH sensitive dye reports the transport. The ease of integrating functional membranes and reporters through the use of patterned mesophases should enable high throughput studies of membrane transport.
Medical subject headings
- Gramicidin
- Ion Channel Gating
- Ion Channels
- Lipid Bilayers
- Membrane Fluidity
- Microscopy, Fluorescence
- Phospholipids