Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.
basic_science · Level V
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- Record sourced from PubMed, PMID 1763053.
- Also identified by PMC identifier 53140.
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Abstract
Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.
Medical subject headings
- Chromosomes, Human
- Ligases
- Mitosis