Tomographic phase microscopy.
basic_science · Level V
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Abstract
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy, Phase-Contrast
- Tomography