One- and two-photon excited optical ph probing for cells using surface-enhanced Raman and hyper-Raman nanosensors.
basic_science · Level V
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Abstract
We demonstrate spatially resolved probing and imaging of pH in live cells by mobile and biocompatible nanosensors using surface-enhanced Raman scattering (SERS) of 4-mercaptobenzoic acid (pMBA) on gold nanoaggregates. Moreover, we also show that this concept of pH nanosensors can be extended to two-photon excitation by using surface-enhanced hyper-Raman scattering (SEHRS). In addition to the advantages of two-photon excitation, the SEHRS sensor enables measurements over a wide pH range without the use of multiple probes.
Medical subject headings
- Biosensing Techniques
- Hydrogen-Ion Concentration
- Microscopy, Fluorescence, Multiphoton
- Nanotechnology
- Spectrum Analysis, Raman