Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.
basic_science · Level V
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- Record sourced from PubMed, PMID 17873058.
- Also identified by PMC identifier 2000531.
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Abstract
In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP(7)) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATP to prime them for IP(7) phosphorylation. IP(7) phosphorylates synthetic phosphopeptides but not if their phosphates have been masked by methylation or pyrophosphorylation. Moreover, IP(7) phosphorylated peptides are more acid-labile and more resistant to phosphatases than ATP phosphorylated peptides, indicating a different type of phosphate bond. Pyrophosphorylation may represent a novel mode of signaling to proteins.
Medical subject headings
- Adenosine Triphosphate
- Inositol Phosphates