MegaPlex PCR: a strategy for multiplex amplification.
other · Level V
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- Record sourced from PubMed, PMID 17873887.
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Abstract
'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.
Medical subject headings
- Polymerase Chain Reaction