Comprehensive analysis of diverse ribonucleoprotein complexes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 17922018.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The study of the dynamic interactome of cellular ribonucleoprotein (RNP) particles has been hampered by severe methodological limitations. In particular, the affinity purification of intact RNP complexes from cell lysates suffers from RNA degradation, loss of interacting macromolecules and poor overall yields. Here we describe a rapid affinity-purification method for efficient isolation of the subcomplexes that dynamically organize different RNP biogenesis pathways in Saccharomyces cerevisiae. Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination.
Medical subject headings
- Ribonucleoproteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins