Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 17982460.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.
Medical subject headings
- Immunoassay
- Microchemistry
- Polymerase Chain Reaction
- Prostate-Specific Antigen