In situ proteolysis for protein crystallization and structure determination.
basic_science · Level V
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- Record sourced from PubMed, PMID 17982461.
- Also identified by PMC identifier 3366506.
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Abstract
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
Medical subject headings
- Crystallization
- Crystallography
- Peptide Hydrolases
- Proteins