Rapid control of protein level in the apicomplexan Toxoplasma gondii.
basic_science · Level V
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- Record sourced from PubMed, PMID 17994029.
- Also identified by PMC identifier 2601725.
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Abstract
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.
Medical subject headings
- Gene Expression Regulation
- Gene Silencing
- Gene Targeting
- Genetic Engineering
- Protozoan Proteins
- Toxoplasma