In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
basic_science · Level V
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Abstract
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
Medical subject headings
- Drosophila melanogaster
- Image Enhancement
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Tomography, Optical