Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging.

Sinha, Tuhin K; Khatib-Shahidi, Sheerin; Yankeelov, Thomas E; Mapara, Khubaib; Ehtesham, Moneeb; Cornett, D Shannon; Dawant, Benoit M; Caprioli, Richard M et al. · Nat Methods · 2008

basic_science · Level V

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Abstract

We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.

Medical subject headings