Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
basic_science · Level V
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- Record sourced from PubMed, PMID 18084299.
- Also identified by PMC identifier 2290002.
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Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
Medical subject headings
- Drosophila Proteins
- Drosophila melanogaster
- Gene Targeting
- Genetic Vectors
- RNA Interference