Three-dimensional nanoscopy of colloidal crystals.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18166070.
- Also identified by DOI 10.1021/nl073164n.
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Abstract
We demonstrate the direct three-dimensional imaging of densely packed colloidal nanostructures using stimulated emission depletion microscopy. A combination of two de-excitation patterns yields a resolution of 43 nm in the lateral and 125 nm in the axial direction and an effective focal volume that is by 126-fold smaller than that of a corresponding confocal microscope. The mapping of a model system of spheres organized by confined convective assembly unambiguously identified face-centered cubic, hexagonal close-packed, random hexagonal close-packed, and body-centered cubic structures.
Medical subject headings
- Colloids
- Crystallization
- Image Enhancement
- Image Interpretation, Computer-Assisted
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Nanostructures