High-density mapping of single-molecule trajectories with photoactivated localization microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18193054.
- Also identified by DOI 10.1038/nmeth.1176.
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Abstract
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.
Medical subject headings
- Image Enhancement
- Membrane Proteins
- Microscopy, Fluorescence