Printing protein arrays from DNA arrays.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18204456.
- Also identified by DOI 10.1038/nmeth.1178.
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Abstract
We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
Medical subject headings
- Gene Expression Profiling
- Oligonucleotide Array Sequence Analysis
- Protein Array Analysis