Gold nanostoves for microsecond DNA melting analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18220441.
- Also identified by DOI 10.1021/nl073028i.
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Abstract
In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.
Medical subject headings
- Biosensing Techniques
- DNA
- Gold
- Nanostructures
- Nanotechnology
- Sequence Analysis, DNA