Single-molecule cut-and-paste surface assembly.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18239119.
- Also identified by DOI 10.1126/science.1151424.
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Abstract
We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency.
Medical subject headings
- DNA
- Microscopy, Atomic Force
- Nanotechnology
- Nucleic Acid Hybridization
- Oligodeoxyribonucleotides