A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae.
basic_science · Level V
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- Record sourced from PubMed, PMID 18246075.
- Also identified by DOI 10.1038/nmeth.1181.
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Abstract
Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.
Medical subject headings
- Gene Expression Profiling
- Gene Library
- Genome, Fungal
- Saccharomyces cerevisiae