Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18264105.
- Also identified by DOI 10.1038/nmeth.1184 and PMC identifier 3439997.
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Abstract
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
Medical subject headings
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA