Tractable Cre-lox system for stochastic alteration of genes in mice.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18264106.
- Also identified by DOI 10.1038/nmeth.1183 and PMC identifier 2394730.
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Abstract
We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.
Medical subject headings
- Adenosine Triphosphatases
- DNA Mismatch Repair
- DNA Repair Enzymes
- DNA-Binding Proteins
- Disease Models, Animal
- Integrases