Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18264107.
- Also identified by DOI 10.1038/nmeth.1182 and PMC identifier 2279183.
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Abstract
Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.
Medical subject headings
- Integrases
- Microsatellite Repeats
- Mosaicism
- Transgenes