Microelectrode array (MEA) platform for targeted neuronal transfection and recording.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18270028.
- Also identified by DOI 10.1109/TBME.2007.914403.
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Abstract
Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.
Medical subject headings
- Microelectrodes
- Neurons
- Transfection