High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18301740.
- Also identified by DOI 10.1371/journal.pone.0001662 and PMC identifier 2244704.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.
Medical subject headings
- Gene Expression Profiling
- Oligonucleotide Array Sequence Analysis