Dynamic temperature measurement in microfluidic devices using thermochromic liquid crystals.

Hoang, Viet N; Kaigala, Govind V; Backhouse, Christopher J · Lab Chip · 2008

basic_science · Level V

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Abstract

Thermochromic liquid crystals (TLCs) are used to explore the temperature transients during thermal cycling for microchip-based polymerase chain reaction (PCR). By analyzing the reflected spectra of the TLCs over time, temperature vs. time trajectories were extracted and overshoots/undershoots were estimated. To our knowledge, this is the first report of TLC-based dynamic temperature measurements in a microfluidic device for all PCR temperature stages.

Medical subject headings