Genetic control of neuronal activity in mice conditionally expressing TRPV1.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18327266.
- Also identified by DOI 10.1038/nmeth.1190 and PMC identifier 3127246.
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Abstract
Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.
Medical subject headings
- Behavior, Animal
- Integrases
- Neurons
- Recombination, Genetic
- TRPV Cation Channels