In vivo immunogold labeling confirms large-scale chromatin folding motifs.
basic_science · Level V
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- Record sourced from PubMed, PMID 18345005.
- Also identified by DOI 10.1038/nmeth.1196.
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Abstract
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA).
Medical subject headings
- Chromatin
- DNA Replication
- Microscopy, Electron