Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18369516.
- Also identified by DOI 10.1039/b716388f.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.
Medical subject headings
- Biosensing Techniques
- Hemolysin Proteins
- Ion Channels
- Lipid Bilayers
- Microfluidic Analytical Techniques
- Polyethylene Glycols