Layer-by-layer assembly of viral capsid for cell adhesion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18387348.
- Also identified by DOI 10.1016/j.actbio.2008.02.026.
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Abstract
Cowpea mosaic virus (CPMV)-based thin films are biologically active for cell culture. Using layer-by-layer assembly of CPMV and poly(diallyldimethylammonium chloride), quantitatively scalable biomolecular surfaces were constructed, which were well characterized using quartz crystal microbalance, UV-vis and atomic force microscopy. The surface coverage of CPMV nanoparticles depended on the adsorption time and pH of the virus solution, with a greater amount of CPMV adsorption occurring near its isoelectric point. It was found that the adhesion and proliferation of NIH-3T3 fibroblasts can be controlled by the coverage of viral particles using this multilayer technique.
Medical subject headings
- Capsid
- Comovirus
- Fibroblasts