Suspended-drop electroporation for high-throughput delivery of biomolecules into cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18408727.
- Also identified by DOI 10.1038/nmeth.1201 and PMC identifier 2668720.
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Abstract
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
Medical subject headings
- Electroporation
- Transfection