Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes.
basic_science · Level V
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- Record sourced from PubMed, PMID 18412403.
- Also identified by DOI 10.1021/nl080358+.
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Abstract
Fluorescence resonance energy transfer (FRET) characteristics, including the efficiency, donor-acceptor distance, and binding strength of six fluorescent protein (FP)-quantum dot (QD) pairs were quantified, demonstrating that FPs are efficient acceptors for QD donors with up to 90% quenching of QD fluorescence and that polyhistidine coordination to QD core-shell surface is a straightforward and effective means of conjugating proteins to commercially available QDs. This provides a novel approach to developing QD-based FRET probes for biomedical applications.
Medical subject headings
- Crystallization
- Fluorescence Resonance Energy Transfer
- Histidine
- Molecular Probe Techniques
- Nanotechnology
- Quantum Dots